Effects of viola-containing serum on phagocytosis and TOLL-like receptor expression of macrophage / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 759-762, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-452737
ABSTRACT
Objective:
To investigate effects of viola on macrophage TOLL-like receptor expression ,and tentatively explore the partial mechanism of viola intervention on macrophage function.Methods:
Using viola water decoction lavage intervention in clean grade SD rats of conventional preparation containing serum ,In a certain concentration coculture with mouse peritoneal macrophages in 96-well plates.After 24 hours,cell phagocytosis activity was determined by neutral red uptake assay ,changes in expression levels of TLR-1,TLR-2,TLR-3,TLR-4,TLR-5 mRNAs were determined by RT-PCR.Results:
Compared with the same concentration of normal serum group(1 ) viola-containing serum group macrophage activity was significantly increased ( P0.05 ) ,and the expression level of TLR-5 was significantly increased in each group ( P<0.01 ).Conclusion:
Viola-containing serum has obvious promoting effect on macrophage phag-ocytosis,the mechanism of which may be related to that this drug regulates and controls part of macrophage TOLL -like receptor expres-sion.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2014
Type:
Article
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