Detection of Synthetic Pigments in Chinese Patent Drugs by Solid Phase Extraction-HPLC / 中国药师

ABSTRACT

Objective:To establish a method for detecting synthetic pigments in Chinese patent drugs by solid phase extraction-HPLC(SPE-HPLC). Methods:Purified with SPE and concentrated, the sample was separated on a SHISEIDO C18 column with 0. 02 mol·L-1 ammonium acetate and methanol mixed solution as the mobile phase with gradient elution at the flow rate of 1. 0 ml·min-1 , the detection wavelength of 508nm, column temperature of 30℃,and the injection volume of 10μl. Results: The linear range of the synthetic pigments was 0. 005-0. 200 μg(r=0. 999 9), and the average recovery was 97. 6%(RSD=1. 3%,n=6),97. 0%(RSD=1. 4%,n=6) and 99. 5%(RSD=1. 4%,n=6), respectively. Conclusion:The proposed method is simple, reliable and reproduci-ble, and especially suitable for the detection of synthetic pigments in Chinese patent drugs.