Determination of Dracorhodin in Gushang Dieda Zhitong Capsules by HPLC / 中国药师

ABSTRACT

Objective:To establish a method to determine dracorhodin in Gushang Dieda Zhitong capsules by HPLC. Methods:An HPLC method was used for the determination of dracorhodin, a Shimadzu VP-ODS C18 column was used,and acetonitrile-0. 05 mol ·L-1 sodium dihydrogen phosphate solution(35∶65)was the mobile phase. The flow rate was 1.0 ml·min-1. The detection wave-length was at 440nm. Results:The linear range of dracorhodin was 0. 047-0. 234 μg(r=0. 999 9). The average recovery was 99. 9%with RSD of 1. 5%. Conclusion:The method is simple, rapid, accurate and specific. It can be used in the quality control of Gushang Dieda Zhitong capsules.