Determination of Content and Encapsulation Efficiency of Genistein-loaded Eudragit Nanoparticles by HPLC / 中国药师
China Pharmacist
; (12): 204-206, 2014.
Article
in Zh
| WPRIM
| ID: wpr-452854
Responsible library:
WPRO
ABSTRACT
Objective:To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods:The HPLC analysis was carried out on a DiamonsilTM C18(250 mm ×4.6 mm,5μm)column with the mobile phase of meth-anol-water (85∶15) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 261nm and the column temperature was 25℃. Results: A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1(r =0.999 8). The recovery of genistein was 99.85%(RSD =0. 65%,n=9). Conclusion:The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles.
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WPRIM
Language:
Zh
Journal:
China Pharmacist
Year:
2014
Type:
Article