Optimal concentration of modified platelet-rich plasma to promote the proliferation of dental pulp stem cells from deciduous teeth / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 4517-4523, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-453006
ABSTRACT
BACKGROUND:
Previous experiments have shown that modified platelet-rich plasma activated by liquid nitrogen freezing and thawing can promote the proliferation of human bone marrow mesenchymal stem cells and dental pulp stem cells in a concentration-dependent manner.OBJECTIVE:
To investigate the effect of modified platelet-rich plasma at different concentrations on the proliferation of dental pulp stem cells from human exfoliated deciduous teeth.METHODS:
Platelets were selected and harvested by automatic blood cellanalyzer, and then activated by liquid nitrogen freezing and thawing.α-MEM served as basal medium. Different concentrations of modified platelet-rich plasma (2%, 5%, 10%, 20%) or 10%fetal bovine serum were added, respectively. The difference in cellproliferation was observed. RESULTS ANDCONCLUSION:
Modified platelet-rich plasma at different concentrations could promote the proliferation of dental pulp stem cells from deciduous teeth. The effects of 2%modified platelet-rich plasma and 10%fetal bovine serum on promoting the proliferation of dental pulp stem cells from deciduous teeth were similar. These results indicated that 2%modified platelet-rich plasma could promote the proliferation of dental pulp stem cells from deciduous teeth, and substitute for fetal bovine serum in the amplification of dental pulp stem cells from deciduous teeth in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2014
Type:
Article
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