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The Effect of Epigallocatechin-3-gallate on HIF-1alpha and VEGF in Human Lung Cancer Cell Line / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases ; : 178-185, 2009.
Article in Korean | WPRIM | ID: wpr-45327
ABSTRACT

BACKGROUND:

Epigallocatechin-3-gallate (EGCG) is the major catechin in green tea, and has shown antiproliferative, antiangiogenic, antimetastatic and cell cycle pertubation activity in various tumor models. Hypoxia can be induced because angiogenesis is insufficient for highly proliferating cancer. Hypoxia-inducible factor-1alpha (HIF-1alpha) and its downstream target, vascular endothelial growth factor (VEGF), are important for angiogenesis, tumor growth and metastasis. The aim of this study was to determine how hypoxia could cause changes in the cellular phenomena and microenvironment in a non-small cell culture system and to examine the effects of EGCG on a HIF-1alpha and VEGF in A549 cell line.

METHODS:

A549 cells, a non-small cell lung cancer cell line, were cultured with DMEM and 10% fetal bovine serum. A decrease in oxygen tension was induced using a hypoxia microchamber and a CO2-N2 gas mixture. Gas analysis and a MTT assay were performed. The A549 cells were treated with EGCG (0, 12.5, 25, 50 micromol/L), and then examined by real-time-PCR analysis of HIF-1alpha, VEGF, and beta-actin mRNA.

RESULTS:

Hypoxia reduced the proliferation of A549 cells from normoxic conditions. EGCG inhibited HIF-1alpha transcription in A549 cells in a dose-dependent manner. Compared to HIF-1alpha, VEGF was not inhibited by EGCG.

CONCLUSION:

HIF-1alpha can be inhibited by EGCG. This suggests that targeting HIF-1alpha with a EGCG treatment may have therapeutic potential in non-small cell lung cancers.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Tea / RNA, Messenger / Catechin / Cell Cycle / Cell Line / Actins / Carcinoma, Non-Small-Cell Lung / Cell Culture Techniques / Vascular Endothelial Growth Factor A Type of study: Prognostic study Limits: Humans Language: Korean Journal: Tuberculosis and Respiratory Diseases Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Tea / RNA, Messenger / Catechin / Cell Cycle / Cell Line / Actins / Carcinoma, Non-Small-Cell Lung / Cell Culture Techniques / Vascular Endothelial Growth Factor A Type of study: Prognostic study Limits: Humans Language: Korean Journal: Tuberculosis and Respiratory Diseases Year: 2009 Type: Article