Determination of hyperoside in Bushen-Yijing wan by Ion-Pair HPLC / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 723-725, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-453336
ABSTRACT
Objective To establish a HPLC method for the determination of Hyperoside in Bushen-Yijing wan. Methods The HPLC system consisted of the Agilent HC-C18(4.6 mm×250 mm, 5μm)column was adopted. The mobile phase was consisted of acetonitrile(10)-HK2PO4(90)(0.85 g/L sodium heptanesulfonate,H3PO4 adjusted pH to 4.8), the flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 360 nm. Results The linear response range was 3.0~60.0μg/ml(r=0.999 9). The average recovery of hyperoside was 98.2%, and RSD1.53%. Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of Hyperoside in Bushen-Yijing wan.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Traditional Chinese Medicine
Year:
2014
Type:
Article
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