Effect of ischemic preconditioning on expression of intracellular adhesion molecule-1 in brain tissues following traumatic brain injury in rats / 中华创伤杂志
Chinese Journal of Trauma
;
(12): 464-466, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-453485
ABSTRACT
Objective To investigate the effect of ischemic preconditioning (IPC) on expression of intracellular adhesion molecule-1 (ICAM-1) in brain tissues following traumatic brain injury (TBI) in rats.Methods Sixty male SD rats weighing 220-250 g were randomly divided into three groups (n =20 for each)sham operation group,TBI group,and IPC group.Cerebral IPC models were induced by transient occlusion of the bilateral common carotid arteries; TBI models were induced by Feeney's freefalling method; rats in sham operation group were only performed exposure of dura of the right parietal lobe.Ten rats were sacrificed respectively at 6 and 72 hours after TBI and injured brain tissues were harvested to estimate wet/dry weight (W/D) ratio for the brain,determine ICAM-1 expression by immunohistochemistry and perform microscopic examination.Results Brain W/D ratio was significantly increased in TBI group compared with sham operation group (6 h4.2 ± 0.4 vs 2.7 ± 0.4 ; 72 h5.0 ±0.1 vs 3.1 ± 0.2,P < 0.05).ICAM-1 expression was up-regulated in TBI group compared with sham operationgroup (6h25.4±3.5vs8.6±1.3; 72 h36.5±5.4 vs8.4±1.6,P<0.05).W/D ratio was significantly decreased in IPC group compared with TBI group (6 h3.5 ±0.6 vs 4.2 ±0.4; 72 h3.7 ± 0.4 vs 5.0 ± 0.1,P < 0.05).ICAM-1 expression was down-regulated in IPC group compared with TBI group (6 h16.5 ± 2.7 vs 25.4 ± 3.5 ; 72 h24.3 ± 4.6 vs 36.5 ± 5.4,P < 0.05).Milder injury to brain tissues was observed in IPC group than in TBI group.Conclusion IPC can attenuate TBI in rats by down-regulating the expression of ICAM-1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Trauma
Year:
2014
Type:
Article
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