Comparison of Several Methods and Their Progresses on Molecular Authentication of Lonicera japonica Thunb / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine
;
(12): 1831-1839, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-453945
ABSTRACT
Authentications of Chinese herbal medicine have a critical effect in Chinese clinical medicine. DNA molecular marker, as an important component for true or false authentication, is more and more widely used in iden-tification of Chinese medicinal materials. At the same time, many new methods for authentication of Chinese medici-nal materials are continuously emerging. But the systematically comparative analysis of these new methods is lack. The present study taking Lonicera japonica as an example, systematically compared principles, characteristics, ex-periment methods, detection time and the application scope of express sequence tag-simple sequence repeat (EST-SSR), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), allele-specific PCR (AS-PCR), DNA barcoding and loop-mediated isothermal amplification (LAMP), and put forward corresponding improve-ment opinions. This study can help to screen appropriate approach for rapid authentication of L. japonica and offer demonstrating to other Chinese herbal medicines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
World Science and Technology-Modernization of Traditional Chinese Medicine
Year:
2014
Type:
Article
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