Effects of Gingerol on Melanogenesis of B16 Melanoma Cells / 医药导报

ABSTRACT

Objective To study the effects of the gingerol on the melanogenesis in melanoma B16 cells. Methods Melanoma cells were cultured with gingerol at 12. 5, 25. 0, 50. 0, 100. 0, 200. 0 μmol · L-1 and positive control drug hydroquinone,respectively,using Dulbecco's modified eagle's medium(DMEM) as the blank control group. The cell proliferation was measured by methyl thiazolyltet tetrazolium ( MTT) colorimetric assay. The tyrosinase activity and melanin content were measured by colorimetry assay. Results Gingerol at different concentrations had inhibitory effect on B16 cell proliferation compared with the blank control group ( P < 0. 05 or P < 0. 01), the inhibition rate being more than 48% at the dosage of 200. 0 μmol·L-1 . Tyrosinase activity was inhibited significantly compared with blank control group(P<0. 05 or P<0. 01),the inhibition rate being up to 50% at the dosage of 200 μmol·L-1 . Melanin content was also decreased at all levels of gingerol compared with blank control group(P<0. 05 or P<0. 01),but not in a dose-dependent manner. The inhibition rate of melanin content reached the plateau at gingerol levels greater than 25. 0 μmol · L-1 . Conclusion Gingerol can inhibit the cell proliferation,tyrosinase activity and decrease melanin synthesis in certain range of concentrations.