Effects of penehyclidine hydrochloride on lipopolysaccharide-induced neonatal cardiomyocyte dam-age and NF-κB activation / 临床外科杂志

ABSTRACT

Objective To observe the effects of penehyclidine hydrochloride(PHC)on lipopo-lysaccharide-induced neonatal cardiomyocyte damage.Methods After the primary cardiomyocytes culture of neonatal rat on postnatal day 1,the myocytes were randomly divided into 4 groupsthe control group,the lipopolysaccharide(LPS)group,the low dosage PHC pretreatment group and high dosage PHC pretreatment group.Activities of myocardial enzymes and cardiac troponin and cell survival rate were measured.Expres-sion of NF-Κb(p65)and IκB-α(NF-κB inhibitor)phosphorylation were assessed using Western blot.Re-sults Compared with the control group,activities of myocardial enzymes and cardiac troponin in the LPS group were elevated but the survival rate were down-regulated.All these effects were reversed in the PHC pretreatment group.Results of Western blot showed that phosphorylation of IκB-αand p65 expression were up-regulated in the LPS group.In the PHC pretreatment group,phosphorylation of IκB-αand p65 expres-sion were down-regulated.Conclusion Penehyclidine hydrochloride can inhibit lipopolysaccharide-in-duced neonatal cardiomyocyte damage and the down-regulation of NF-κB activity might be involved.