Genotyping of Escherichia coli isolated from ICU and drug resistance situation analysis / 国际检验医学杂志

ABSTRACT

Objective To explore the drug resistance and genotypes in 30 strains of multi-drug resistant Escherichia coli isolated from ICU patients to provide the basis for rational use of antibacterial drugs in ICU patients .Methods The MIC method was a-dopted to analyze the antimicrobial susceptibility of Escherichia coli and the Enterobacteriaceae intergenic repetitive sequence (ERIC)-polymerase chain reaction (PCR) method was adopted to perform the genotyping of multi-drug resistant Escherichia coli screened out by the antimicrobial susceptibility .Results 30 strains of Escherichia coli were isolated from ICU and divided into two genotypes of type A and B2 ,among which 16 strains were isolated in sputum (5 strains of type B2) ,9 strains in blood (7 strains of type B2) ,3 strains in lung lavage fluid and 2 strains in urine (1 strain of type B2) .The drug susceptibility test results showed 13 strains of type B2 and their resistance rates to amoxicillin ,cefuroxime ,piperacillin ,ticarcillin were over 90% .17 strains were type A and their resistance rates to amoxicillin ,cephalothin ,gentamicin ,ticarcillin and piperacillin reached more than 70% .The multi-drug resistance in type B2 was higher than that in the type A .Conclusion Type B2 strain of Escherichia coli shows multi-drug resist-ance and strong pathogenicity and mainly distributes in the blood samples .The use of antibacterial drugs in ICU patients should be reasonably adjusted according to different genotypes of Escherichia coli infection .