Changes of melatonin and melatonin receptors on 6-OHDA induced Parkinson's disease in rat / 中华行为医学与脑科学杂志

ABSTRACT

Objective To observe the changes of dopamine and its metabolites,melatonin,as well as melatonin receptors in the substantia nigras (SN) in 6-OHDA induced hemiparkinsonian rats.Methods PD rat was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta and medial forebrain bundle.Rats were divided into PD groups and vehicle groups,and sacrificed at 1000 and 2200 respectively,at 3 and 6 weeks post-lesion.The contents of dopamine and its metabolites as well as melatonin were measured by HPLC.The expressions of MT1 and MT2 mRNA and protein were detected by RT-PCR and Western-Blot respectively.Results Dopamine levels declined by 83％ and 88％ respectively at 3 and 6 weeks post-lesion in the injected side of PD rats,compared with the contralateral side.Melatonin levels in the injected side increased by 22％ and 29％ respectively.However,melatonin increased by 46％ and 31％ respectively at 1000 and 2200,at 6 weeks post-lesion in the contralateral side,compared with the same side of the vehicle.The expressions of MT1 and MT2 receptors mRNA were significantly decreased by 91.04％ and 90.24％ respectively in comparison with the vehicle,while the protein expression of MT1 and MT2 receptors significantly declined by 58％ and 70％ respectively.Conclusion Increased melatonin and down-regulated MT1 and MT2 receptors in the lesioned SN in 6-OHDA-induced rats may be associated with the progression of PD.