Effects and mechanism of penhyclidine hydrochloride on myocardial ischemia reperfusion injury in rat / 中国综合临床

ABSTRACT

Objective To explore effects and mechanism of penhyclidine hydrochloride (PHC) on myocardial ischemia reperfusion injury in rat.Methods Sixty Sprague Dawley (SD) adult male rats were randomly divided into sham operation group(SHAM group),myocardial ischemia reperfusion injury group(MIRI group) and penhyclidine hydrochloride treatment (PHC group),and 20 rats in every group.Compared left ventricular function of 3 groups after 24 h of ischemia reperfusion.Selected 10 rats of every group and compared the myocardial infarction size after ischemia reperfusion,and the less 10 rats' the level of tumor necrosis factor-α (TNF-αt) and nuclear factor-kB(NF-kB) in myocardial tissue were measured.Results After 24 h of ischemia reperfusion,there was no significant myocardial infarction size in SHAM group,while that in MIRI group were (39.4± 3.7)％,higher than that in PHC group (20.5 ± 2.3)％,and the difference was significant (P ＜ 0.01).The level of left ventricular end-systolic volume (LVESV) of rats in SHAM,MIRI and PHC groups were (0.138 ± 0.024),(0.457 ± 0.032) and (0.280 ± 0.027) ml respectively,left ventricular end-diastolic volume(LVEDV) were (1.012 ± 0.065),(1.395 ± 0.076) and (1.183 ± 0.102) ml respectively,and left ventricular ejection fraction (LVEF) were (85.6 ± 7.3) ％,(43.2 ± 4.5) ％ and (64.8 ± 5.1) ％ respectively,and there were statistically significant differences between groups (F =54.12,32.70,35.46,P ＜ 0.001).The level of TNF-αtand NF-kB in rats of SHAM,MIRI and PHC group were (5.4 s0.7),(34.2 ±2.9) and (21.6 ± 1.3) pg/mg respectively and (87.6 ± 7.3),(134.2 ± 9.6) and (116.3 ± 8.7) respectively,and there were statistically significant differences between groups (F =59.40,17.97,P ＜ 0.001).There were statistically significant differences between MIRI and SHAM groups,PHC and SHAM groups in terms of above factors (P ＜0.05).Conclusion PHC can decrease the inflammatory reaction and protect the myocardial ischemia reperfusion injury in rat.