Investigation and analysis on carrying nuc-mec A gene situation in outpatient and inpatient departments / 国际检验医学杂志

ABSTRACT

Objective To investigate the carrying situation of nuc-mec A gene for different departments in the inpatient depart-ment and outpatient department to provide the data for the hospital infection control and reaching the target for preventing nosoco-mial infection and improving the cure rate.Methods The research subjects were divided into 4 groups:osteoarthropathy and hemi-plegia rehabilitation department group(A,44 cases),comprehensive surgical and hematology-oncology department group(B,32 ca-ses),cardiopulmonary department group(C,17 cases)and outpatient department group(D,35 cases).The group A,B and C were the persons in the inpatient department and the group D were the persons in the outpatient department,aged 30-84 years old.Na-sal swab samples were collected and the nuc-mec A gene was detected by the multi-channel real-time PCR.Results No person in the group A,B and C carried nuc gene and 1 case(2.86%)in the group D carried nuc gene.There were no statistically significant differ-ence among 4 groups(P >0.05);the carriers of mecA gene in the group A,B,C and D were 26,17,13 and 10 cases,the carrying rates were 59.10%,53.10%,76.50% and 28.60% respectively,the difference showed statistical significance(P 0.05);the carrying rate of nuc-mec A gene showed statistically significant differences between the patients and the medical staff(P 0.05).Conclusion nuc-mec A gene generally exists in the inpatient de-partment and the carrying rate of nuc-mec A gene is relatively lower,which indicates that the inpatient department needs to pay at-tention to the prevention and treatment of pathogenic bacteria,especially the de-clonization in the hemiplegia rehabilitation and car-diopulmonary departments for avoiding cross-infection among different departments.