Determination of Rutin in Anoectochilus Roxburghii by HPLC / 中国药师
China Pharmacist
;
(12): 1584-1585, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-456290
ABSTRACT
Objective:
To establish a method for the determination of rutin in Anoectochili roxburghii.Methods:
The detection was carried out by HPLC. A Lanbo-Kromasil C18 column(250 mm × 4. 6 mm,5 μm) was used with methanol-0. 2% phosphoric acid solu-tion (40∶60) as the mobile phase;the detection wavelength was 257nm, the flow rate was 1. 0 ml·min-1 and the column temperature was 30℃.Results:
Rutin showed a good linear relationship within the range of 0. 021-0. 433 μg(r=0. 999 6). The average recovery was 96. 65%(RSD=1. 67%, n=9).Conclusion:
The method is simple, rapid, specific and reproducible.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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