Determination of Compound Sulfur Ointments by UPLC / 中国药师

ABSTRACT

Objective:To establish a method for the determination of salicylic acid in the compound sulfur ointments by ultra-per-formance liquid chromatography (UPLC). Methods:An Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5 μm)column was used, the mobile phase was methanol-0. 1 mol·L-1 sodium dihydrogen phosphate (30∶70), the detection wavelength was 231nm, the column temperature was 35℃, the flow rate was 1. 0 ml·min-1 and the injection volume was 10μl. Results:The linear range of salicylic acid was 11. 96-59. 80 μg·ml-1(r=0. 999 9), and the average recovery was 99. 72%(RSD=1. 28%,n=9). Conclusion:The method is accurate, stable and specific, and suitable for the determination of salicylic acid in the compound sulfur ointments.