Determination of Benzo(a) pyrene Residues in Edible Oil and Oil Products by High Performance Liquid Chromatography with Dispersive Solid Phase Extraction / 分析化学

ABSTRACT

A dispersive solid phase extraction method combined with reversed phase liquid chromatography was investigated for the determination of benzo ( a ) pyrene ( BaP ) residues in edible oil and oil products. The developed “one-step” sample preparation method integrated with the adsorbent activation, sample extraction and sample cleaning. Using Alumia-N as the adsorbent, good cleaning effect and high recovery ranging from 81. 5% -97. 5% was achieved. In addition, coupled with fluorescence detector (FLD), high sensitivity was obtained with LOD of 1. 0 μg/kg (S/N=3). Furthermore, SPSS was used to design orthogonal optimization experiments and establish the optimal condition. Under the optimized condition, the standard calibration curve was linear over the concentration range of 0. 5-10. 0μg/L, with the regression efficiency (R2) of 0. 9997. The relative standard deviation (RSD) of peak area was between 2. 6%-4. 9%, showing good repeatability and the reliability.