Bioinformatics analysis of lung cancer metastasis-related miR-29b / 中国肿瘤临床
Chinese Journal of Clinical Oncology
;
(24): 1021-1025, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-456503
ABSTRACT
Objective:
This paper aims to bioinformatically analyze the target genes of miR-29b and to provide clues for cancer research targeting miR-29b.Methods:
The differential expression levels of miRNAs in CD133+and CD133-A549 cells were detected using the miRNA PCR chip. Real-time polymerase chain reaction was performed to verify the partially differential expression of miR-NAs. Target genes of miR-29b were predicted by miRecords and analyzed by gene ontology and signal transduction pathway enrich-ment analysis.Results:
The miR-29b expression was significantly decreased in the CD133+A549 cells compared with that in the CD133-cells. The number of miR-29b target genes was 106. The functions of these target genes were enriched in binding and extracel-lular matrix structural constituent (P<0.01). The JAK-STAT and TGF-βsignal transduction pathways were significantly enriched (P<0.05).Conclusion:
The abnormal expression of miR-29b may be related to metastasis. Some of the predicted target genes of miR-29b were significantly enriched in the signaling pathways in relation to the tumors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Clinical Oncology
Year:
2014
Type:
Article
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