Lentivirus mediated CCN1 gene on growth and migration of rat bone marrow mesenchymal stem cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 1493-1496, 2014.
Article
in Zh
| WPRIM
| ID: wpr-456611
Responsible library:
WPRO
ABSTRACT
[ABSTRACT]AIM:Toinvestigatetheroleofcysteine-rich61(Cyr61/CNN1)inproliferationandmigrationof bone marrow mesenchymal stem cells ( BMSCs ) .METHODS: The lentiviral vector carrying CCN 1 ( Lenti-GFP-CCN1 ) was constructed and then transfected into the rat BMSCs .The cells were divided into non-transfection group , transfection group ( transfected with Lenti-GFP-CCN1 ) and negative control group ( Lenti-GFP ) .The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope .The effects of CCN1 on the proliferation and mi-gration of BMSCs were detected by MTT assay and scratch wound healing assay .RESULTS:The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group .The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05).CONCLUSION:Exogenous CCN1 promotes the migration of BMSCs.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
2014
Type:
Article