Role of miRNA-24 in regulation of endothelial nitric oxide synthase ex-pression and vascular endothelial cell proliferation / 中国病理生理杂志

ABSTRACT

[ABSTRACT]AIM:ToinvestigatewhethermiRNA-24isinvolvedintheregulationofendothelialnitricoxide synthase ( eNOS ) expression and vascular endothelial cell proliferation .METHODS: A plasmid that highly expressed miRNA-24 was constructed, and was transfected into the human umbilical vein endothelial cells (HUVECs) by liposome. The cell proliferation was detected by MTT assay .The expression of eNOS and Sp 1 at mRNA and protein levels was exa-mined by real-time PCR, immunohistochemistry and Western blotting .RESULTS:Compared with control group , the pro-liferation of endothelial cells in miRNA-24 group was significantly decreased by 41.97 % (0.47 ±0.04 vs 0.81 ±0.03, P<0.01), and the expression of eNOS at mRNA and protein levels was decreased by 44.8% (0.48 ±0.01 vs 0.87 ± 0.03, P<0.05) and 71.92%(0.16 ±0.06 vs 0.57 ±0.08, P<0.05), respectively.Meanwhile, the mRNA and pro-tein levels of Sp1 were significantly decreased by 53.00% (0.45 ±0.02 vs 0.93 ±0.01, P<0.05) and by 62.31%(0.13 ±0.07 vs 0.31 ±0.09, P<0.05), respectively.In miRNA-24 inhibitor group, the above indexes were decreased compared with control group , but significantly increased compared with miRNA-24 group.CONCLUSION: miRNA-24 significantly inhibits the proliferation of HUVECs and the eNOS expression .Sp1 possibly acts as one of the important factors in the regulation of eNOS expression by miRNA-24.