Changes of NO and iNOS expression in lung tissues and injury of lungs in traumatic shock rats of early stage preconditioned with heat stress / 安徽医科大学学报

ABSTRACT

Objective To research nitric oxide( NO) concentration, induced nitric oxide synthase( iNOS) mRNA expression and the pathological changes of lung tissue in traumatic shock rats of early stage preconditioned with heat stress. Methods A total of 98 male SD rats were equally divided randomly into heat stress untreated group and heat stress treatment group, and each group were equally divided into 7 subgroup: control group and shock 0 h group, 0. 5 h group, 1. 0 h group, 1. 5 h group, 2. 0 h group, 3 h group. To established the model of traumatic shock after heat stress pretreatment and remained lung tissue, observed the changes of pulmonary pathology, meas-ured the changes of the NO concentration and iNOS expression in lung tissues. Results The lesions of lung tissue structure were more lighter in heat stress treatment group than the corresponding time point of heat stress untreated group and lung histopathology score lower. The NO concentration of heat stress treatment group were lower than the corresponding time point of heat stress untreated group, the two groups have a rise at shock 0 h,the peak value of NO concentration in heat stress treatment group appeared later than the heat stress untreated group. INOS mRNA expression quantity of heat stress treatment group were lower than the corresponding time point of heat stress un-treated group, the peak value of heat stress untreated group in 2.0 h and heat stress treatment group in 1. 5 h, it was positively correlated with lung injury score. Conclusion Heat stress pretreatment can drop NO concentration and iNOS mRNA expression in lung tissues significantly, delayed and reduced the lung injury in traumatic shock rats of early stage.