UHPLC determination of paclitaxel in polydipeptide paclitaxel / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 703-706, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-457398
ABSTRACT
Objective To establish an ultra-high-performance liquid chromatograph(UHPLC)method for the determination of paclitaxel (PTX)in polydipeptide paclitaxel (PDP)preparation. Methods PDP preparation was dissolved in deionized water (DIW) and degraded by 2.0 mol/L sodium hydroxide solution. The concentration of paclitaxel was calculated indirectly by its degradation product. The separation was achieved on an Agilent SB C18 column(2.1 mm × 50 mm,1.8μm). Elution was carried out using a mobile phase consisting of acetonitrile-water (10 ∶ 90,V/V)at the flow rate of 0.2 ml/min. UV detection wavelength was performed at 240 nm and reference wavelength was 360 nm. The temperatures of autosampler and column were thermostated at 15℃(± 0.5℃)and 40℃(± 0.5℃),respectively. The injection volume was 2 μl. Results The relationship between the concentration of paclitaxel (0.31-5.00 mg/ml)and the peak area of its degradation product was in good linearity (r=0.9992,n=5). Total amount of paclitaxel in different batches of PDP preparation was in the range of 26.77-33.19 mg per vial. Conclusion The method is accurate, rapid,reproducible and suitable for the analysis of paclitaxel in PDP preparation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of International Pharmaceutical Research
Year:
2014
Type:
Article
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