Effect of Different Sample Solvents on Determination of Astragaloside Ⅳ by High Performance Liquid Chromatograph_Evaporative Light Scattering Detector / 医药导报

ABSTRACT

Objective To exPlore the effect of samPle_solVent comPositions on the determination of AstragaliⅣby high Performance liquid chromatograPh_ eVaPoratiVe light scattering detector ( HPLC_ELSD ) . Methods Radix astragali and ganduqing granules serVed as samPles. Methanol,90%methanol,80%methanol,70%methanol,32%acetonitrile,15%acetonitrile, and water were samPle solVents. HPLC_ELSD was used to determine content of astragalosideⅣ. Results The results showed that the 90%methanol solution was an aPProPriate samPle solVent with good system suitability,Precision,accuracy,and,linearity etc. . Conclusion This method benefits the quality control of astragalosideⅣin Radix astragali and agents containing Radix astragali.