To explore the method of establishing hepatocellular steatosis models by Changliver cell / 重庆医学

ABSTRACT

Objective To explore the experimental condition for hepatocellular steatosis models of Changliver cell induced by o‐leic acid (lleic acid ,OA) .Methods Changliver cells were induced by different concentration of oleic acid for different periods .MTT was used to detect hepatic cell activity ,oil red 0 staining was used to observe intracellular lipid droplets acumulation ,glycerin 3 phosphate oxidase method was applied to detect the contents of triglyceride (TG) in the Changliver cell .Results Hepatocellular steatosis models of Changliver cell can be established successfully by 0 .2 mmol/L OA inducing for 24 hours .TG content in model cells was (379 .98 ± 23 .19)mg/g ,however ,it was (185 .03 ± 12 .68)mg/g in control cells ,the difference was statistically significant (P< 0 .01) .Conclusion The proper condition for establishing hepatocellular steatosis models is 0 .2 mmol/L OA inducing Changliver cells for 24 h .This model is the reliable choice for nonalcoholic fatty liver disease research .