Expression of Musashi 1 correlates with microvessel density and prognosis in colon cancer / 中国免疫学杂志

ABSTRACT

Objective:To investigate the Musashi 1 expression, and its correlation with clinical variables, such as clinical pathologic factors,angiogenesis and prognosis in colon cancer.Methods: Musashi 1 mRNA level was determined by quantitative real-time PCR( qRT-PCR) in 36 pairs of matched colon cancer tissues and adjacent non-tumorous tissues.The expression of Musashi 1 and mi-crovascular density ( MVD) were tested by immunohistochemical in 96 cases of colon cancer.Combined with postoperative follow-up survival situation, the correlation between the expression of Musashi 1, pathological parameters, prognosis and MVD were statistical analylised.Results:Musashi 1 mRNA level of colon cancer tissues determined by PCR was significantly higher than adjacent non-tumorous tissues( P<0.05).Positive Musashi 1 immunoreactivity was seen in 63.5%of colon cancer specimens,which was correlated with the depth of invasion,Duckes stage,lymph node metastasis,and TNM stage (P<0.05).Compared with Musashi 1 negative patients, Musashi 1 positive patients had significantly shorter survival time ( 23.0% vs 60.0%, P<0.01 ) .High intratumor blood microvessel density patients had significantly shorter survival times than low intratumor blood microvessel density patients(P<0.05).Intratumor blood microvessel density correlated with Musashi 1 expression (P<0.01).Conclusion:Musashi 1 mRNA level of colon cancer tissues was higher than adjacent non-tumorous tissues,the difference has statistically significant.We conclude that Musashi 1 expression correlates with the depth of invasion,Dukes stage,lymph node metastasis,and TNM stage and microvascular density ( MVD) in colon cancer.We propose that synthesized Musashi 1 increases intratumor angiogenesis,thus promotes tumor progression.Musashi 1 expression may be a good biomarker for poor prognosis in colon cancer.