Determination of Two Components and Preservative in Compound Dextromethorphan Hydrobromide Syr-ups by HPLC / 中国药师
China Pharmacist
;
(12): 2056-2058, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-458784
ABSTRACT
Objective:
To establish an HPLC method for the determination of two components and the preservative in compound dextromethorphan hydrobromide syrups.Methods:
An Agilent Zobax SB-C18 column(250 mm × 4. 6 mm,5 μm) was used with meth-anesulfonic acid solution (adding 4. 8g methanesulfonic acid and 10ml triethylamine into 750ml water,and adjusting the pH value to 3. 5 by phosphoric acid)-acetonitrile (75∶25) as the mobile phase at the flow rate of 1. 0 ml·min-1 and 280nm as the detection wave-length.Results:
The calibration curve was linear within the range of 102-1 025μg·ml-1 for guaifenesin,15-619μg ·ml-1 for dextro-methorphan hydrobromide and 10-407μg·ml-1 for benzoic acid. The average recovery of guaifenesin, dextromethorphan hydrobromide and benzoic acid was 100. 0% (RSD=0. 35%), 100. 1%(RSD=0. 77%)and 100. 8%(RSD=0. 49%), respectively.Conclusion:
The method is simple,rapid and accurate,and suitable for the quality assessment of compound dextromethorphan hydrobromide syrups.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS