Protective effect of peperphentonamine hydrochloride on the gentamicin-induced cochlear afferent nerve system damage of guinea pigs / 实用医学杂志
The Journal of Practical Medicine
;
(24): 3045-3048, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-459808
ABSTRACT
Objective To study the protective effect of peperphentonamine hydrochloride (PPTA) on the cochlea afferent nerve system damage induced by gentamicin (GM) of guinea pigs. Methods Thirty-six health guinea pigs were randomly divided into three groupsthe normal group with saline; GM group with muscle injection of GM [100 mg/(kg·d),14 d] and PPTA group with peritoneal injection of PPTA [10 mg/(kg·d),14 d] on the ABR was analyzed , and the expression of Caspase-3 was tested by Western blot. TUNEL (after coloration)was performed to observe the apoptotic index,and its morphological changes were observed by TEM. Results After trial, the threshold of control group, GM group, PPTA group were 14.58 ± 1.16, 65.95 ± 1.17, 36.13 ± 1.17;the apoptosis index 1.09 ± 0.14, 23.17 ± 0.88, 8.84 ± 0.49; the Caspase-31.09 ± 0.11, 2.55 ± 0.20, 1.67 ± 0.07. There were significantly difference among three groups (P<0.05). Morphological changes cells apoptosis and the synapase were damaged seriously in GM group; the injury was less serious in PPTA group. Conclusion PPTA plays a protective role on GM-induced cochlea afferent nerve system damage of guinea pigs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
The Journal of Practical Medicine
Year:
2014
Type:
Article
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