Study on Methodolgy of Content Determination of Three Flavonoids in Traditional She Medicine Shi Liang Cha / 中国药师
China Pharmacist
;
(12): 1808-1810, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-460046
ABSTRACT
Objective:
To establish an HPLC method for the simultaneous determination of three flavonoids in Shi Liang Cha.Methods:
HPLC was applied with the chromatographic conditions as follows the chromatographic column was Agilent Zorbax SB-C18 (250 mm × 4. 6 mm, 5μm) at 30℃;acetonitrile-0. 1% H3 PO4 solution was used as the mobile phase with gradient elution; the flow rate was 1. 0 ml·min-1, and the detection wavelength was 360nm.Results:
Good linearity of rutin, quercetin and kaempferol was within the range of 0.0409-1.637 0mg·ml-1(r=0.999 2), 0.44-88.00μg·ml-1(r=0.999 8) and 0.41-77.63μg·ml-1(r=0. 999 2), respectively; the average recovery was 99.35%(RSD =1. 64%),101. 14% (RSD =1. 88%) and 99. 69% (RSD =1. 92%) , respectively.Conclusion:
The method is simple, accurate and repeatable, and suitable for the simultaneous determination of rutin, quercetin and kaempferol in Shi Liang Cha.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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