Simultaneous Content Determination of Gatifloxacin Dexamethasone Acetate Ear Drops by HPLC / 中国药师
China Pharmacist
;
(12): 1969-1971, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-460073
ABSTRACT
Objective:
To developed an HPLC method for the simultaneous content determination of gatifloxacin dexamethasone ac-etate ear drops.Methods:
The promising chromatographic separation was achieved on a Phenomenex C18 (250 mm × 4. 6 mm, 5 μm) column. Using gradient elution method, the optimized mobile phase consisted of methanol as the solvent A, and 0. 05% triethylamine solution (adjusting pH to 3. 0 with acetic acid) as the solvent B. The flow rate was 1. 0 ml·min-1, the column temperature was 30℃and the UV detection was carried out at 240 nm.Results:
The compounds were separated well, the linearity between the peak areas and the concentrations was observed within the range of 1. 2-60. 0 mg·L-1 for gatifloxacin, and 0. 8-40. 0 mg·L-1 for dexamethasone acetate. The mean recovery of gatifloxacin and dexamethasone acetate was 101. 31% ( RSD =1. 17%) and 99. 71% ( RSD =1. 01%) , respectively.Conclusion:
The method is specific and stable in the determination of gatifloxacin dexamethasone acetate ear drops.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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