An Analysis of the Diversification of Cell Temperature of Photothermal Effects of Gold Nanorods Coated with mAb-EGFR on FADU Human Pharyngeal Squamous Cell Lines and Non-tumor Cells 293 T Cell Line / 听力学及言语疾病杂志

ABSTRACT

Objective To study the cell temperature on photothermal effects of gold nanorods coated with mAb-EGFR targeted on FADU human pharyngeal squamous cell lines and non- tumor cells(293T cell line) .Meth‐ods CTAB gold nanorods were synthesized to complete the functional modifications of EGFR monoclonal antibody , with a UV spectrophotometer and electron microscopy characterization .The cultured FADU hypopharyngeal cells and non-tumor cells 293 T cell lines were expressed by western blot EGFR statutory comparison of two cell lines , cells to swallow the gold nanorods were observed .Cell apoptosis was observed by Annexin-V -FLUOS and pro‐pidium iodide .Then we set different doses of gold nanoparticles concentration (15% ,25 % ,and 35% ) .After 6 minutes'near-infrared laser irradiation (λ=808 nm) ,the temperature was recorded once every minute ,and cell viability was detected by XTT assay .Results After six minutes'near -infrared laser irradiation ,the cell tempera‐ture was 41 ℃ ~43 ℃ ,with 15% ~25 % of gold nanorods .The cell killing effects on hypopharyngeal were signifi‐cant .The 293T cells did not show any damaging effects (P<0 .05) ,whereas the concentration of gold nanoparticles in 35% of hypopharyngeal FADU cells and 293T cells had significant cytotoxicity (P<0 .05) .Conclusion There were significant cell killing effects with 15% ~25% gold nanorods coated with mAb-EGFR in the near -infrared laser irradiation six minutes (Cell temperatures of both cell lines were 41 ℃ ~43 ℃)on hypopharyngeal FADU cell but not on non-tumor cells .