Simultaneous Determination of Five Active Components in Sijiganmao Tablets by HPLC / 中国药师
China Pharmacist
;
(12): 487-490, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-460394
ABSTRACT
Objective:
To develop an HPLC method for the simultaneous determination of five active components ( 5-O-methyl-visammioside, prim-O-glucosycimifugin, hesperidin, rosmarinic acid and forsythin) in Sijiganmao tablets.Methods:
The samples were separated on an Agilent C18 column (250 mm × 4. 6 mm,5 μm) by acetonitrile-0. 1% formic acid solution with gradient elution. The detection wavelength was 283nm,the flow rate was 1. 0μl·min-1 ,the column temperature was 30℃,the detection wavelength was 283 nm,and the sample size was 10μl.Results:
The complete separation was obtained for the five active compounds. The five regression e-quations showed good linear relationships. The average recoveries of the compounds were between 95. 0% and 105. 0%.Conclusion:
The established method is accurate, reliable, simple and effective, which can be used in the quality control of Sijiganmao tablets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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