B cells activation stimulated by autophagosomes derived from tumor cells / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 354-357, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-460406
ABSTRACT
Objective:
To study B cells proliferation and activation induced by tumor derived-autophagosomes ( TDA).Methods:
Splenocytes of mice were incubated with 10μg/ml TDAs in vitro ( with the 10μg/ml whole cell lysate as the control).At the 3rd day,the expression of MHCⅠ,Ⅱand co-stimulate molecules including CD86,CD40 were detected by flow cytometry,and B cells proliferation was detected at the 5th day.B cells were selected from spleen of mice using anti-CD43 dynabeads,and incubated with 10μg/ml TDA in vitro ( with the same concentrated whole tumor cell lysate as the control).At the 7th day,IgM in the supernatant were tested by ELISA.Results:
When compared with whole cell lysate stimulated B cells,TDA efficently stimulated B cells division in vitro ( TDA group28.6%, Whole cell lysate group4.4%) , and significantly up-regulated the expression of MHC class Ⅰ,Ⅱ and co-stimulatory molecules (CD86 and CD40) on B cells,and enhanced the levels of total IgM secretion in vitro.Conclusion:
Tumor-derived autophagosomes ( TDA) ,as a vacuole antigen vector, could stimulate B cells proliferation, activation, and increased IgM secretion in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2015
Type:
Article
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