Role of hypoxia-inducible factor 1 alpha and inducible nitric oxide synthase in neural stem cells during hypoxia/reoxygenation injury / 中国组织工程研究

ABSTRACT

BACKGROUND:Organism in the hypoxic state can produce hypoxia-inducible factor, but nitric oxide generated from inducible nitric oxide synthase can inhibit the activity of hypoxia-inducible factor 1α. OBJECTIVE:To discuss the relationship of hypoxia-inducible factor 1α and inducible nitric oxide synthase with hypoxia/reoxygenation injury in neural stem cels. METHODS:Under sterile conditions, Wistar rats born within 24 hours were sacrificed to separate the rat hippocampus that was used to prepare a cel suspension of brain tissue. After culture and passage, neural stem cels were divided into normoxia, hypoxia and hypoxia/reoxygenation groups. In the latter two groups, 150 μmol/L cobalt chloride solution was used to prepare hypoxia models, and in the hypoxia/reoxygenation group, the cels were reoxygenated after 4-hour hypoxia. RESULTS AND CONCLUSION:Under hypoxic conditions, a significant increase in mRNA expressions of hypoxia-inducible factor 1α and inducible nitric oxide synthase as wel as the number of apoptotic neural stem cels. Compared with the hypoxia group, the number of apoptotic neural stem cels was higher in the hypoxia/reoxygenation group, but the mRNA expressions of hypoxia-inducible factor 1α and inducible nitric oxide synthase were lower. These findings indicate that these two factors are involved in the hypoxia/reoxygenation injury of neural stem cels.