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Preparation and measurement of sodium tanshinone Ⅱ-A sulfonate in mice serum and effects on big conductance calcium-activated potassium channels in porcine coronary artery smooth muscle cells / 重庆医学
Chongqing Medicine ; (36): 436-438, 2015.
Article in Chinese | WPRIM | ID: wpr-460876
ABSTRACT
Objective To discuss a method combining serumpharmacology and electrophysiology technology ,and to research the mechanism of dilating porcine coronary artery of sodium tanshinone Ⅱ‐A sulfonate (DS‐201) .Methods To give mice intragastric administration solution and measure DS‐201 concentration in mice serum ,and apply the serum to single channel patch to research its effect on big conductance calcium‐activated potassium channels(BKca ) in porcine coronary artery smooth muscle cells (PCASMCs) . Results The linear range of concentration containing DS‐201 serum was 0 .73 to 1 .91 μg/mL (r=0 .997 7) ,the recycle rate was 99 .85% -101 .47% ,and the concentration was(7 .32 ± 4 .25)μg/mL ;the result indicates that serum containing DS‐201 has activa‐tion effects on BKCa in PCASMCs ,while there was no statistical significance (P>0 .05) .Conclusion The establishment method of the alcohol extraction of Danshen is useful and reliable .The HPLC method of measuring DS‐201 concentration is precise .Choosing higher quality drugs or raising bioavailability can improve combination of the serum pharmacology and electrophysiological tech‐nique .

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chongqing Medicine Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chongqing Medicine Year: 2015 Type: Article