Stem Cell Tracking with Amphiphilic Polyethyleneimine Modified Quantum Dots as Functional Optic Probes / 华中科技大学学报(医学版)
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
;
(6): 28-31, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-461002
ABSTRACT
Objective Stem cell therapy has been extensively used in clinical practice. However ,the methods available are unable to non‐invasively detect the distribution of cells in vivo. This study was aimed to establish a novel molecular imaging probe for stem cell labeling ,which would monitor cells via fluorescence imaging. Methods 1‐iodododecane was used to modify polyethyleneimine (PEI) to form amphiphilic macromolecules. These macromolecules encapsulated the hydrophobic quantum dots by self‐assembly to form imaging probes. Results Strong fluorescence signals of the probe occurred at 630 nm ,and the probe could be effectively endocyted by mesenchyme stem cells. In vivo imaging showed that significant optic signals occurred in probe‐labeled mesenchyme stem cells compared with the control cells (blank group1.47e8 [p/s/cm2/sr]/[μW/cm2 ];test group2.78e8 [p/s/cm2/sr]/[μW/cm2 ]). Conclusion The nanoprobes have good optic imaging effects ,and can be used to la‐bel cells.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
Year:
2015
Type:
Article
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