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S, R-Isomer Separation of Hydrochloride Palonosetron by RP-HPLC with HP-β-CD as Chiral Additive / 中国药师
China Pharmacist ; (12): 237-240, 2015.
Article in Chinese | WPRIM | ID: wpr-461073
ABSTRACT

Objective:

To establish an HPLC method for the separation of optical isomers of palonosetron hydrochloride by RP-HPLC with HP-β-cyclodextrin ( HP-β-CD) as the chiral additive.

Methods:

The optimal separation process was performed on a Shi-seido CAPACELL PAK C18 VG 120 (250 mm × 4. 6 mm,5μm) column,the mobile phase was triethylamine (TEA) with HP-β-CD at different concentrations, the pH value was adjusted by acetic acid, the detection wavelength was 240nm, and the column temperature was 30℃.

Results:

The mobile phase was 0. 5% TEA (1% HP-β-CD, adjusting pH to 5. 5 with acetic acid)-acetonotirile (85∶15);the detection wavelength was 240nm, and the flow rate was 0. 5 ml·min-1 . The S, R-Isomer of palonosetron hydrochloride could be well separated.

Conclusion:

The method can be used as the optical isomer quality control for palonosetron hydrochloride.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2015 Type: Article