S, R-Isomer Separation of Hydrochloride Palonosetron by RP-HPLC with HP-β-CD as Chiral Additive / 中国药师
China Pharmacist
;
(12): 237-240, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-461073
ABSTRACT
Objective:
To establish an HPLC method for the separation of optical isomers of palonosetron hydrochloride by RP-HPLC with HP-β-cyclodextrin ( HP-β-CD) as the chiral additive.Methods:
The optimal separation process was performed on a Shi-seido CAPACELL PAK C18 VG 120 (250 mm × 4. 6 mm,5μm) column,the mobile phase was triethylamine (TEA) with HP-β-CD at different concentrations, the pH value was adjusted by acetic acid, the detection wavelength was 240nm, and the column temperature was 30℃.Results:
The mobile phase was 0. 5% TEA (1% HP-β-CD, adjusting pH to 5. 5 with acetic acid)-acetonotirile (85∶15);the detection wavelength was 240nm, and the flow rate was 0. 5 ml·min-1 . The S, R-Isomer of palonosetron hydrochloride could be well separated.Conclusion:
The method can be used as the optical isomer quality control for palonosetron hydrochloride.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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