Determination of P-aminophenol in Pediatric Paracetamol and Amantadine Hydrochloride Granules by HPLC / 中国药师

ABSTRACT

Objective:To establish an HPLC method for the determination of P-aminophenol in pediatric paracetamol and amanta-dine hydrochloride granules. Methods: The column was Phenomenex Luna C8 (150 mm × 4. 6 mm, 5 μm), the mobile phase was phosphate buffer solution-methanol (93∶7), the flow rate was 0. 8 ml·min-1, the detection wavelength was 275nm, the column tem-perature was 30℃, and the injection volume was 10μl . Results: P-aminophenol showed good linearity within the range of 0. 528-42. 240 μg·ml-1(r=0. 999 9). The average recovery was 100. 03%(RSD=0. 9%, n=9). Conclusion:The method is simple with high accuracy and reproducibility, which can be used in the determination of P-aminophenol in pediatric paracetamol and amantadine hydrochloride granules.