Simultaneous Determination of Two Components in Aspirin and Phenobarbital Powders by HPLC / 中国药师
China Pharmacist
;
(12): 343-345, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-461124
ABSTRACT
Objective:
To establish an HPLC method for the determination of two components in aspirin and phenobarbital pow-ders.Methods:
AThermoBDSC18column(250mm×4.6mm,5μm)wasusedwithacetonitrile-methanol-0.01mol·L-1KH2PO4so-lution -triethylamine(10∶35∶55∶0.1)(adjusting pH to 3.5 with phosphoric acid )as the mobile phase. The flow rate was 1.0 ml· min-1 and the detection wavelength was 242nm. The column temperature was ambient and the injection volume was 20 μl.Results:
There was a good linear relationship when the content of aspirin and phenobarbital was within the range of 0. 402 0 ~1. 608 0 mg· ml-1(r=0.999 7) and 0.061 2 ~0.244 8 mg·ml-1(r =0.999 6), respectively. The average recovery was 99.21%(RSD =0. 49%,n=9) and 99. 63% (RSD=0. 39%,n=9), respectively.Conclusion:
The method is simple and accurate with good repro-ducibility, and can be used to control the quality of the preparation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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