Chemical constituents of ethanol extracts from Daphne altaica pall and their pro-apoptotic role in cancer cell / 天津医药

ABSTRACT

Objective To investigate the chemical constituents of the ethanol extract of Daphne altaica Pall (DA-Et), to determine total phenolic content in DA-Et and to study the effect of DA-Et on cell apoptosis in Eca-109 cells. Meth?ods Different chemical assays were performed to detect chemical constituents of DA-Et. The content of total phenolics ,ex?pressed as gallic acid equivalents,was measured by Folin-Ciocalteu assay; Cell apoptosis induced by DA-Et was detected by AnnexinV-FITC/PI flow cytometry. Results Flavonoids,coumarins,sugars,alkaloids and phenolics in DA-Et. The contents of total phenolics in DA-Et was quantified as 159.78 mg/g. Average recovery rate of this method ranged from 99.4% to 108.3% and the precision relative standard deviation was 2.04%. Apoptosis rate of Esophageal cancer cell line Eca-109 induced by DA-Et was detected as 29.633%±1.779%(n=3). Conclusion Our study provide guidance for the im?provement of DA-Et quality, its appropriate application and further research of its activity.