Influence of regulation of host cell autophagy on the proliferation of Toxoplasma gondii in host cells / 安徽医科大学学报

ABSTRACT

Objective To study the role of autophagy in the replication process of Toxoplasma proliferation. Meth-ods As the experimental groups, these cells were infected by Toxoplasma gondii tachyzoites at given MOI (2 ∶ 1, 4 ∶ 1 ,8 ∶ 1 ,16 ∶ 1 ) . Host cell autophagy was detected through acridine orange staining and MDC fluorescence stai-ning at different time points (1, 2, 4, 8, 24, 48, 96 hs post infection). Detect the condition of HEF cells autoph-agy with acridine orange fluorescence staining and MDC fluorescence staining, and detect the replication kinetics of Toxoplasma gondii infection at different time points using Giemsa staining. Results The results of acridine orange and MDC fluorescence staining showed that autophagy inhibitors and inducers could inhibit and promote the autoph-agy of HEF cells respectively. From the results of Giemsa staining, it was found that the proliferation of Toxoplasma gondii in HEF cells could be promoted with autophagy inducers and be inhibited with autophagy inhibitors. Conclu-sion The regulation on autophagy of host cell could regulate the proliferation and replication of Toxoplasma gondii.