Effect of immunity-related GTPase Irgm1 gene knockout on CD4+T cell subsets in experimental autoimmune encephalomyelitis / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 180-184, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-461983
ABSTRACT
Objective:
To investigate whether Irgm 1 impact CD4+T cell subsets in the experimental autoimmune encephalomyelitis.Methods:
The Irgm1 heterozygous mice were backcrossed with C 57BL/6 Wt.mice for 10 generations to produce C57BL/6 Irgm1+/-mouse.C57BL/6 Irgm1+/-mice were intercrossed to obtain three genotypesIrgm1-/-, Irgm1+/-and Irgm1+/+.To establish model of EAE ,C57BL/6 Wt.mice and Irgm1 knock out mice were immunized with myelin oligodendrocyte glycolprotein 33-55 ( MOG33-55 ) and the clinical symptoms were observed.The proliferation of lymphocytes to MOG antigen was detected with Methyl Thiazolyl Tetrazolium ( MTT).The infiltration of inflammatory cells in the spinal cords was observed through HE staining.The CD4+T cell subsets from lymph nodes ,spleens and CNS were detected by flow cytometry.Results:
EAE model was induced successfully.The proliferation of T cells in lymph nodes in Irgm 1-/-mice was lower than Wt.mice.Quantitative analysis of flow cytometry indicates that , compared with Wt.mice,the level of Th1 subset was higher,Th17 was lower relatively in lymph nodes and CNS of Irgm1 knock out mice.Conclusion:
Irgm1 knockout mice can be partially protected EAE spinal cord function and clinical symptoms .Irgm1 may play a key role at early stage of EAE ,which may use as an important molecular target for treatment of EAE.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS