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Comparison of osteopontin and mineralization culture medium in the induction of dental pulp stem cells into osteoblasts / 实用口腔医学杂志
Journal of Practical Stomatology ; (6): 11-14, 2015.
Article in Chinese | WPRIM | ID: wpr-462080
ABSTRACT

Objective:

To compare the osteogenic differentiation of dental pulp stem cells(DPSCs)induced by osteopontin(OPN)and mineralizing culture medium(MCM).

Methods:

DPSCs were cultured with OPN(OPN group)and MCM(MCM group)respectively. The morphology of the DPSCs were observed under inverted microscope.The mineralize nodules were observed by alizarin red staining. RT-RCR was used to detect the mRNA expression of bone sialoprotein (BSP),Runt-related transcription factor 2(Runx-2),osteocal-cin(OCN)and collagen-1(Col-1).

Results:

Similar number of mineralized nodules was found in the 2 groups(P >0.05)after 28 day culture.The mRNA expression level of BSP gene in OPN group was higher than that in MCMgroup(0.864 ±0.112 and 0.514 ±0.068, P 0.05).

Conclusion:

The capabilities of OPN and MCMin inducing osteogenic differentiation of DPSCs are similar.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2015 Type: Article