Comparison of osteopontin and mineralization culture medium in the induction of dental pulp stem cells into osteoblasts / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 11-14, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-462080
ABSTRACT
Objective:
To compare the osteogenic differentiation of dental pulp stem cells(DPSCs)induced by osteopontin(OPN)and mineralizing culture medium(MCM).Methods:
DPSCs were cultured with OPN(OPN group)and MCM(MCM group)respectively. The morphology of the DPSCs were observed under inverted microscope.The mineralize nodules were observed by alizarin red staining. RT-RCR was used to detect the mRNA expression of bone sialoprotein (BSP),Runt-related transcription factor 2(Runx-2),osteocal-cin(OCN)and collagen-1(Col-1).Results:
Similar number of mineralized nodules was found in the 2 groups(P >0.05)after 28 day culture.The mRNA expression level of BSP gene in OPN group was higher than that in MCMgroup(0.864 ±0.112 and 0.514 ±0.068, P 0.05).Conclusion:
The capabilities of OPN and MCMin inducing osteogenic differentiation of DPSCs are similar.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2015
Type:
Article
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