Evaluation of combined detection of antibodies specific for syphilis / 国际检验医学杂志

ABSTRACT

Objective To evaluate ELISA combined with chemiluminescence in the detection of syphilis specific antibodies. Methods 628 patients who came to our hospital and treated in dermatology department were recruited in the study from January 2014 to April 2014.Patients′serum samples were detected by using ELISA firstly,and then the samples,the test rusults of which were within the recheck standard range(0.5chemiluminescence.And then the results of parallel Treponema pallidum gelatin agglutination test(TPPA)measurement were compared with.Results There were 171 cases of TPPA positive samples detected and 162 cases meets the positive criteria for syphilis (S/CO≥3.0)in the hospital.In addition to that,15 cases of syphilis detected were within the recheck standard range.In the samples rechecked by using chemiluminescencea,there were 9 cases of positive results,and 6 cases of negative results.171 cases of positive results were detected by the combined method,which were consistent with TPPA.Conclusion The detection results of ELISA combined with chemiluminescence detection are consistent with that of TPPA.