Chitosan microspheres loading whole cell protein antigen ofHelicobacter pylori: preparation and in vitrorelease characteristics / 中国组织工程研究

ABSTRACT

BACKGROUND: Studies on encapsulated whole cel protein antigen ofHelicobacter pylori are stil at the exploration stage. There is limited literature concerning the preparation process andin vitro release characteristics of chitosan microspheres encapsulated with whole cel protein antigen ofHelicobacter pylori. OBJECTIVE:To explore the preparation process andin vitrorelease characteristics of chitosan microspheres encapsulating whole cel protein antigen ofHelicobacter pylori. METHODS: Precipitation method was used to prepare chitosan microspheres, and the best preparation process, matching and encapsulation time were screened. Under electron microscope, the morphology and particle size of microspheres were observed. Chitosan microspheres were used to encapsulateHelicobacter pylori whole cel protein antigen, and BCA method was used to determine encapsulation efficiency, encapsulation content and release efficiency in vitro of Helicobacter pylori whole cel protein antigen. RESULTS AND CONCLUSION:Final concentration of 1% glacial acetic acid, sodium sulfate as crosslinking agent, pH=5.0, with no pulverization when the crosslinking agent was added was the best preparation process for chitosan microspheres. Electron microscopy showed the smooth surface morphology of microspheres with roundness and good dispersion, and the majority of the microspheres were 1-5 μm in diameter. The encapsulation efficiency ofHelicobacter pylori whole cel protein antigen microspheres was 80.4%, the encapsulated amount was 16.4%, and total 48-hour release rate was 19.4%.Helicobacter pylori whole cel protein antigen microspheres showed an overal slow release status. Chitosan microspheres show good encapsulation efficiency and amount ofHelicobacter pylori whole cel protein antigen, and Helicobacter pylori total bacteria protein antigen microspheres show an overal slow release status.