Effects of New Compound Traditional Chinese Medicine Prepatations Banxiao Capsule on the Mobilization of Endothelial Progenitor Cells / 医学研究杂志

ABSTRACT

Objective To investigate the effects of new compound traditional Chinese medicine prepatations Banxiao capsule on the mobilization of endothelial progenitor cells ( EPCs) .Methods After EPCs from mouse bone marrow treated with variable dose of Banxiao capsule, we detected the proliferation by CCK-8 assay, quantified the number of CD34/Flk-1 double positive cells by flow cytometric analysis, evaluated the migratory function of EPCs by transwell chamber assay, and studied the protein level of Bcl-2/Bax by Western blot analysis.Results Compared with untreated group, the proliferation potential of EPCs in Banxiao low dose (20μg/ml) and high dose (200μg/ml) group was increased(P<0.05), the number of CD34/Flk-1 double positive cells were increased (P<0.05), migrative activity of EPCs was increased (P<0.05),the level of Bcl-2 protein were increased, and the level of Bax protein were decreased.Con-clusion Banxiao capsule can enhance proliferation of EPCs through up-regulating of expression of Bcl-2/Bax.