Effects of naomaiyihao capsule on survival of bone marrow messchymal stem cell transplantaion / 医学研究生学报

ABSTRACT

Objective Nowadays ,in the field of stem cell transplantation ,improving the bone mesenchymal stem cells′ability to be implanted into target organs is still a hot topic .Objective of this study is to observe the effects of Naomaiyihao ( NMYH) capsule, a compound traditional Chinese herbal medicine invented for regulating thesea of blood in brain,and survival rate of BMSCs trans-plantaion in cerebral ischemic tissue of rat . Methods 60 rats were randomly divied into 4 groupuntreated group ( serum-free L-DMEM +isotonic saline ) , BMSCs transplantation group ( cell suspension+isotonic saline ) , NMYH group ( serum-free L-DMEM+NMYH) and Combination of NMYH and BMSCs group ( cell suspen-sion+NMYH ) .A rat of model of middle cerebral artery occlusion ( MCAO) was established .The third generation BMSCs were isolated and cultured from bone marrow by the holo-bone marrow adherence , the CM-Dil labeled BMSCs were transplanted into corpora striata with stereotactic technique , the rat′s brains were removed and sliced up after 7-,14-and 21-day′transplantation .Then the survival and distribution of cells were observed under the fluorescence microscope . The expression of CD34 in the brain tissue were tested by immunohistochemical method . Results The fluorescence absorbance of combination group was 1.61 ±0.25 on day 14, increased significantly (P<0.05).when compared with BMSCs group (1.23 ±0.17). The fluorescence absorbence of combination group was 0.36 ±0.09 on day 21, increased significantly (P<0.05) when compared with BMSCs group(0.23 ±0.09).Compared with the model group ,numbers of CD34 positive cells on day 7,14 and 21 significantly in-creased in all treated groups(P<0.01).Numbers of CD34 positive cells were significantly more increased(P<0.05) in combination group than those in BMSCs group . Conclusion Survival,distribution and migration trending to boundary regions of ischemia were observed under the fluorescence microscope after transplantation .Naomaoyihao can increase blood supply around the transplanted cell region,and decrease infarct volume .Therefore it could promote BMSCs to better survive in the brain tissue .