Effect of programmed death-1 on human osteosarcoma cells MG-63 / 中国生化药物杂志

ABSTRACT

Objective To discuss the influences of programmed death-1 (PD-1) factor on osteosarcoma cells MG-63.Methods Osteosarcoma stem cells were sovted and identified through osteosarcoma cell strain MG-63 cells.The influence of PD-1 signal on T cells proliferation were detected by MTT. The expression of PD-1 mRNA was detected by RT-PCR.Results Cancer cells had an obvious proliferation within one week, which showed the strong ability of proliferation and aggressivity.The formation of tumor cells spheres depended on the support of serum nutrition.The number of MG-63 cells proliferation in serum culture medium was significantly higher than the osteosarcoma cells spheres in serum-free suspension culture ( P<0.05 ) .Pluripotent stem cell marks that the expression of CD133 in cancer cell sphere was significantly higher than that of MG-63(P<0.05).RT-PCR results showed the PD-1 expression level of cancer cell sphere and MG-63 were increased significantly.Conclusion MG-63 cell line has the character istics of osteosarcoma stem cells.MG-63 cell line can express the corresponding cell markers.The expression of PD-1 also increase significantly which can reduce immune function of patients and is closely related with the occurrence and development of tumors.