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IKKαregulates ultraviolet radiation-induced activation of p53 in a p38K-dependent manner / 军事医学
Military Medical Sciences ; (12): 321-324, 2015.
Article in Zh | WPRIM | ID: wpr-463524
Responsible library: WPRO
ABSTRACT
Objective To explore the signal transduction mechanism of inhibitor kappa B kinase α( IKKα) , one of the catalytic subunits of IKK complex , for regulating p53 transactivation in the cellular ultraviolet radiation ( UVB) repsonse. Methods The transactivation of p53 was determined by dual-luciferase reporter gene analysis system while the expression and activation of IKKα, IKKβ, p53 and p38K was detected by Western blotting assay .Results UVB exposure induced activation and transactivation of p 53 in the wild type mouse fibroblasts ,but the effect was blocked by IKKa deficiency and recovered by reconstitution of IKKαexpression.Under the same conditions , IKKαregulated p38K activation, while inhibi-ting p38K activation down-regulated p53 transactivation under UVB exposure .Conclusion IKKαregulates UVB-induced phosphorylation and activation of p 53 in a p38K-dependent manner .
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Full text: 1 Index: WPRIM Language: Zh Journal: Military Medical Sciences Year: 2015 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Military Medical Sciences Year: 2015 Type: Article