The expression of BMP-2 mRNA of rat osteoblast cell sheets cultured with ascorbic acid / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 305-308, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-463595
ABSTRACT
Objective:
To study the effects of ascorbic acid on BMP-2 mRNA expression of osteoblast cell sheets.Methods:
Rat os-teoblasts were primaryly cultured and identified;osteoblast cell sheets were built by physical scraping method in vitro;the osteoblast cell sheets were cultured with 1 5,50 and 85 mg/L ascorbic acid for 1 and 2 weeks respectively,and the expression of BMP-2 mRNA of the cell sheets was detected by RT-qPCR.Results:
The obtained cells were conformed to be osteoblasts.The osteoblast cell sheets could be rolled into tube in vitro.The expression of BMP-2 mRNA of osteoblast cell sheets in experiment group,whether in week one or week two was higher than that in control group,50 mg/L group showed the highest expression(first week P 0.05);the expression of any group in week two was higher than that in week one(P <0.05).Conclusion:
Ascorbic acid may pro-mote the expression of BMP-2 mRNA in osteoblast cell sheets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2015
Type:
Article
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